Journal: Frontiers in Endocrinology

Article Title: Growth Hormone Overexpression Disrupts Reproductive Status Through Actions on Leptin

doi: 10.3389/fendo.2018.00131

Figure Lengend Snippet: Serum levels of (A) Gh, (B) estradiol, (C) Igf1, (D) Igf2, and (E) glucose contents in female (F) and male (M) non-transgenic (NT) and GH-transgenic (T) common carp. The results obtained were analyzed within sex by independent sample t -test. The P -values are indicated on the graph.

Article Snippet: Igf1 (CSB-E12122Fh) and Igf2 (CSB-EL011088FI) ELISA kits were purchased from Cusabio Life Science (Wuhan, China) ( ).

Techniques: Transgenic Assay

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Trial design and vaccine procedures

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: Infection, Sterility, Injection

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Multiple sequence alignments of IMP1 and AMA1 from different species. a Multiple alignment of E. stiedae IMP1 with IMP1 proteins from other parasites: Eimeria maxima (UniProt: F4MKA6), Eimeria acervulina (UniProt: U6GHD9), Eimeria tenella (UniProt: F4MKA7), Eimeria mitis (UniProt: U6JVY4), Eimeria praecox (UniProt: U6GBP2); ( b ) multiple alignment of E. stiedae AMA1 with AMA1 proteins from other parasites: Eimeria tenella (UniProt: U6KTA0), Eimeria brunetti (UniProt: U6LBB9), Toxoplasma gondii (UniProt: B6KAM0), Besnoitia besnoiti (UniProt: A0A2A9MBX4), Neospora caninum (UniProt: F0VH85); Blue shading indicates conserved residues. Dashed red boxes represent B-cell epitopes. The transmembrane region is marked with a solid red box

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: Sequencing

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: SDS-PAGE and Western blotting analysis of r Es IMP1 ( a ) and r Es AMA1 ( b ). Lane M: Protein molecular weight markers; lanes 1–4: recombinant proteins that were dissolved in the supernatant, 4 M urea, 6 M urea, and 8 M urea after ultrasonication; lane 5: purified recombinant proteins; lane 6: purified recombinant proteins incubated with anti- E. stiedae positive sera; lane 7: purified recombinant proteins incubated with negative sera from coccidia-free rabbits (the bands are indicated using arrows)

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: SDS Page, Western Blot, Molecular Weight, Recombinant, Purification, Incubation

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Protective effects of r Es AMA1 and r Es IMP1 against E. stiedae infection under different evaluation indicators

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: Infection

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Gross postmortem examinations of the liver. a PBS-uninfected group; ( b ) PBS-infected group; ( c ) Quil-A-infected group; ( d ) Trx-His-S-infected group; ( e ) r Es AMA1 group; ( f ) r Es IMP1 group

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: Infection

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Biochemical estimation of ALT and AST levels in the six groups

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques:

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Changes in serum antibody levels. The changes in serum anti-r Es IMP1 ( a ) and r Es AMA1 ( b ) antibody levels after the first vaccination (week 0, indicated by arrows), booster vaccination (week 2, indicated by arrows), and infection with E. stiedae (week 4, indicated by an arrow)

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: Infection